Grape seeds

(Photo by Maryna Osadcha on Shutterstock)

In A Nutshell

  • Researchers examined 1,768 ancient grape seeds from 25 sites to trace the history of grape cultivation in Italy over 7,000 years.
  • The study indicates that Italians relied on wild grape gathering for millennia, with evidence of true grape domestication emerging only during the Late Bronze Age (ca. 1300–1100 BC).
  • Findings highlight that the shift from foraging to organized viticulture was a gradual and regionally diverse process, influenced by trade networks and evolving agricultural practices.

MONTPELLIER, France — Archaeological evidence from 1,768 ancient grape seeds scattered across Italy tells a surprising story: the transition from wild grape gathering to actual winemaking took thousands of years longer than previously thought, with true domesticated grapes not appearing until around 1300 BC.

Led by Mariano Ucchesu at the University of Montpellier in France, scientists analyzed nearly 1,800 waterlogged grape seeds from 25 archaeological sites spanning 7,000 years of Italian history, from the Early Neolithic period (6th millennium BC) through medieval times. Using sophisticated geometric analysis, they compared the ancient seeds to modern wild and domesticated grape varieties to determine exactly when humans transitioned from simply gathering wild grapes to actively cultivating the sweeter, larger fruit we know today.

For most people today, wine feels like an ancient tradition that stretches back to the dawn of civilization. But new research published in PLOS ONE reveals that Italians didn’t actually start cultivating domesticated grapes until much later than expected—and the process was far more gradual and complex than anyone imagined.

Italian wine grapes
The domestication of grapevine was a slow process in Italy, according to the study. (Credit: Jill Wellington, Pexels, CC0 (https://creativecommons.org/publicdomain/zero/1.0/))

Thousands of Years of Wild Grape Gathering

During the Early Neolithic period (around 6000 BC) and continuing through the Early Bronze Age (roughly 2050-1850 BC), archaeological sites contain exclusively wild grape seeds. For thousands of years, early Italians were simply foraging for wild grapes rather than cultivating them.

“During the Early Neolithic, no evidence of morphologically domesticated grapes was observed,” the researchers wrote. Even into the Middle Bronze Age (1600-1300 BC), wild grape varieties still dominated, with only four seeds out of 142 analyzed showing domestic characteristics, a number so small it could represent natural variation or measurement error.

Chemical analysis of Bronze Age ceramics from sites across Italy has revealed wine residues, indicating that people were producing fermented beverages during this period. However, wine can be made from wild grapes too, so ceramic evidence alone doesn’t prove domestication.

Changes in grape pips length from the 25 studied archaeological sites
Changes in grape pips length from the 25 studied archaeological sites, categorized into seven chronological periods, distinguishing seeds according to their classification into wild and domestic types, and comparison to current wild and domestic pips. (Credit: Ucchesu et al / PLOS One)

The Bronze Age Breakthrough

The real turning point came during the Late Bronze Age, around 1300-1100 BC, when archaeological evidence from the site of Sa Osa in Sardinia shows a dramatic shift. Suddenly, 45% of grape seeds displayed domestic characteristics, indicating that communities had finally mastered grape cultivation and selective breeding.

This timing coincides with significant social and political developments in Bronze Age Italy, when hierarchical societies were emerging and trade networks were expanding across the Mediterranean. These complex societies may have provided the stability and knowledge transfer necessary for successful agricultural innovation.

Recent genetic analysis of grape seeds from Sardinia revealed they shared genetic markers with modern Armenian cultivars. This suggests that Bronze Age traders likely brought domesticated grape varieties from the Middle East to Italy, where they gradually spread northward through extensive trade networks connecting Italy with Crete, Cyprus, and other eastern Mediterranean civilizations.

Roman Innovation and Medieval Perfection

By the Iron Age (roughly 800-100 BC), Etruscan sites in central Italy showed predominantly domestic grape varieties, reflecting sophisticated viticulture practices that would later influence Roman wine production. Historical sources confirm that Etruscans were already exporting wine throughout the Mediterranean by the 7th century BC.

Roman sites (1st-6th centuries AD) revealed an interesting pattern: while most grape seeds showed domestic characteristics, some sites contained significant numbers of wild-type seeds alongside domestic ones. Roman vintners were actively experimenting with wild grape cultivation, possibly breeding wild and domestic varieties to create new wine types.

During Roman times, the differences between wild and domestic grape seeds became less pronounced, suggesting that Romans were cultivating various types of vines at different stages of domestication. This experimentation appears to have ended by medieval times (8th-14th centuries AD), when archaeological sites show almost exclusively domestic grape varieties with characteristics virtually identical to modern wine grapes.

A bottle of Chianti at a vineyard in Tuscany, Italy
A bottle of Chianti at a vineyard in Tuscany, Italy (Kishivan/Shutterstock)

A 7,000-Year Agricultural Evolution

The research reveals that grape domestication wasn’t a single revolutionary moment but rather a 7,000-year process of gradual agricultural sophistication. Early Neolithic peoples likely gathered wild grapes seasonally, Bronze Age communities began experimenting with cultivation, Romans perfected breeding techniques, and medieval vintners established varieties that still influence Italian wine production today.

The study’s methodology — comparing seed shape and size using advanced geometric analysis — captures unprecedented precision in tracking these changes. Domestic grape seeds are characteristically larger and more elongated than their wild counterparts, with longer “beaks” at one end. These differences, invisible to the casual observer, tell the story of thousands of years of human selection for sweeter, larger fruit.

This archaeological detective work demonstrates that major agricultural innovations often require not just technological knowledge but also stable societies capable of long-term planning and investment. It’s a lesson that remains relevant for understanding how human civilization develops.

Disclaimer: This summary is based on findings published in PLOS ONE. Quotes are taken verbatim from the paper. Interpretations and speculation about trade, cultural exchange, and agricultural practices are consistent with the authors’ discussion but should not be read as definitive proof of specific historical events.


Paper Summary

Methodology

Researchers analyzed 1,768 waterlogged grape seeds from 25 Italian archaeological sites dating from the Early Neolithic (6th millennium BC) to medieval times (8th-14th centuries AD). They used geometric morphometric analysis, specifically elliptic Fourier transforms, to compare the shape and size of ancient seeds with modern reference collections of wild and domestic grapes. This technique translates seed outlines into mathematical descriptors that can be statistically analyzed. The researchers also measured seed length manually and used linear discriminant analysis to classify seeds as wild, domestic, or intermediate types based on their morphological characteristics.

Results

The study found no evidence of domesticated grapes during the Early Neolithic and Early Bronze Age periods, with all seeds classified as wild types. The first domestic grapes appeared during the Middle Bronze Age (1600-1300 BC) but remained rare. A significant transition occurred during the Late Bronze Age (1300-1100 BC), particularly at the Sa Osa site in Sardinia, where 45% of seeds showed domestic characteristics. By the Iron Age, Etruscan sites showed predominantly domestic varieties. Roman sites displayed a mix of domestic and wild-type seeds, suggesting continued experimentation with grape breeding. Medieval sites contained almost exclusively domestic varieties similar to modern grapes.

Limitations

The study acknowledges several limitations, including incomplete geographical coverage of Italy and temporal gaps in the archaeological record. The research focused primarily on waterlogged seeds, which preserve better than charred specimens but may not represent all ancient grape usage. The classification method, while highly accurate (95.7% correct classification rate), still has margins of error that could affect interpretation of small sample sizes. Additionally, the study cannot definitively distinguish between local domestication processes and the introduction of already-domesticated varieties from other regions.

Funding and Disclosures

The research was funded by the European Union Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement No 101019563 (VITALY project). Additional support came from the ANR MICA project (grant agreement ANR-22-CE27-0026). The authors declared no competing interests and confirmed that all data supporting their conclusions are included in the paper and supplementary materials.

Publication Information

Ucchesu, M., Ivorra, S., Bonhomme, V., Pastor, T., Aranguren, B., Bacchetta, G., et al. (2025) “Tracing the emergence of domesticated grapevine in Italy.” PLOS ONE 20(4): e0321653. Published April 23, 2025. The study is available as an open-access article under Creative Commons Attribution License terms.

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