Fungal mycelium (Mycorrhizae) that provide symbiotic relationship between plants and fungi. (Photo by paitoon Meetee on Shutterstock)
In A Nutshell
- Fungi added to wheat crops helped boost zinc levels in grain and made the nutrient easier for the human body to absorb.
- Iron absorption potential also improved, even though iron levels in the grain didn’t increase overall.
- The treatment worked best in five of eight wheat varieties tested, especially under low-phosphorus soil conditions.
- This natural approach may offer a low-cost way to improve global nutrition without changing farming practices or modifying genes.
ADELAIDE, South Australia — The secret to fighting global malnutrition might be hiding in the soil beneath wheat fields. Australian scientists discovered that when farmers add specific fungi to wheat crops, the grain absorbs significantly more zinc, and delivers it in a form that the human body can more easily use. Iron absorption also improved, even though iron levels in the grain didn’t increase overall.
Around the world, 2 billion people don’t get enough zinc, while 4.5 billion lack sufficient iron in their diets. Both deficiencies cause serious health problems: stunted growth in children, weakened immune systems, and dangerous complications during childbirth. Since wheat provides about one-sixth of these nutrients for many people globally, any improvement could save lives.
The breakthrough, published in the journal Plants, People, Planet, centers on arbuscular mycorrhizal fungi, which are microscopic organisms that naturally partner with plant roots. When researchers added these fungi to eight different wheat varieties, zinc levels increased significantly while a nutrient-blocking compound called phytate stayed the same or even decreased.
What Are Mycorrhizal Fungi and How Do They Help Plants?
These soil fungi operate like an underground trading network. They latch onto wheat roots and spread thin threads throughout the soil, gathering nutrients that plants can’t reach on their own. In return, the wheat feeds the fungi sugars. More than 80% of plants on Earth form these partnerships, including virtually every major food crop.
When soil nutrients are scarce, this relationship becomes especially valuable. Phytate acts like a nutrient thief in the human digestive system: it binds to zinc and iron, preventing the body from absorbing them. Farmers face a frustrating problem: phosphorus fertilizers boost crop yields but also increase phytate levels, making the grain less nutritious even as it becomes more abundant.
How Scientists Tested Fungi on Eight Wheat Varieties
Scientists grew eight popular Australian wheat varieties in controlled greenhouse conditions, comparing plants treated with Rhizophagus irregularis fungi to untreated controls. They tested each variety under both low and high phosphorus fertilizer conditions across 192 individual pots.
After three months of growth, researchers harvested the mature grain and analyzed it using advanced techniques. They measured mineral content with specialized chemical analysis and created detailed maps showing exactly where zinc accumulated within each grain using X-ray technology.
Root examinations revealed that the fungi successfully colonized up to 70% of root length when soil phosphorus was low, dropping to about 40% when phosphorus was abundant. The fungi naturally reduce their efforts when nutrients become readily available.
Fungal Treatment Boosts Zinc Levels and Crop Yields
Five wheat varieties (Calibre, Mace, Rockstar, Scepter, and Trojan) showed increased zinc levels regardless of soil conditions when partnered with fungi. The remaining varieties responded positively under specific fertilizer conditions. One variety, Gladius, achieved particularly impressive zinc concentrations of 19.26 milligrams per kilogram.
Beyond nutrition, the fungi also boosted productivity. Grain weight increased 7–10% in some varieties, particularly under low-phosphorus conditions. Scepter and Spitfire varieties showed the most dramatic yield improvements when treated with fungi.
Most importantly, the fungal partnerships broke the usual trade-off between phosphorus fertilization and mineral absorption. Even when researchers added high levels of phosphorus fertilizer, plants with fungal partners maintained better zinc-to-phytate ratios compared to untreated plants. In several wheat varieties, the fungi sharply improved this ratio, a key factor that affects how well the human body can absorb nutrients. In some cases, the improvement was large enough to suggest that bioavailable zinc levels may have doubled.
Could Fungi-Enhanced Wheat Help Fight Global Malnutrition?
Commercial fungal products already exist for various agricultural uses, though their effectiveness varies based on soil conditions and environmental factors. The researchers used a commercially available product containing about 800 fungal spores per gram of soil.
Different wheat varieties responded uniquely to fungal treatment, which means farmers need to match specific crops with appropriate fungal partners. Some varieties formed extensive partnerships immediately, while others showed more modest colonization rates.
For populations that rely heavily on wheat and other grains, particularly in developing countries with limited meat consumption, even small improvements in mineral absorption could yield significant health benefits. Iron deficiency anemia alone affects over one billion people worldwide and contributes to reduced brain development and increased death rates.
Even in wealthier countries where wheat products provide 20–25% of dietary zinc intake, enhanced mineral absorption could help address hidden deficiencies that impact immune function and overall health without obvious symptoms.
Rather than requiring genetic modification or dramatic changes to farming practices, this approach harnesses biological partnerships that have existed for millions of years. As researchers continue investigating these fungal networks, naturally enhanced nutrition in staple crops moves closer to becoming a practical reality for addressing one of humanity’s most persistent challenges.
Paper Summary
Methodology
Researchers at the University of Adelaide conducted a controlled greenhouse experiment using eight Australian wheat varieties. Plants were grown in sterilized soil mixed with sand, with half receiving inoculation with Rhizophagus irregularis mycorrhizal fungi and half serving as controls. Each variety was tested under both low and high phosphorus fertilizer conditions (0 and 25 mg P per kg soil). The experiment included 192 pots total with six biological replicates per treatment. Plants grew for 81-95 days until maturity, when grain was harvested and analyzed using specialized chemical analysis for mineral content and commercial assay kits for phytate levels. Advanced X-ray fluorescence microscopy mapped nutrient distribution within grain sections.
Results
Mycorrhizal inoculation increased whole grain zinc concentrations in five of eight wheat varieties under both phosphorus conditions, with remaining varieties showing benefits under specific conditions. Grain biomass increased 7-10% in some varieties under low phosphorus when inoculated with fungi. Importantly, fungal partnerships did not increase phytate levels despite higher phosphorus uptake, leading to improved zinc and iron bioavailability ratios. X-ray imaging confirmed higher zinc concentrations in the nutrient-rich aleurone layer of fungal-inoculated grains. Root colonization averaged 66% under low phosphorus and 40% under high phosphorus conditions across all varieties.
Limitations
The study was conducted under controlled greenhouse conditions which may not reflect field agriculture complexities. Only one fungal species was tested, and results may vary with different mycorrhizal strains or environmental conditions. Growing two plants per pot could have influenced results through competition or shared fungal networks. The bioavailability estimates used established ratios rather than actual human digestion studies. Long-term effects and scalability to commercial agriculture remain unknown.
Funding and Disclosures
The research was supported by AgriFutures Australia (Grant PRO-017382), Australian Nuclear Science and Technology Organisation (Grant 21663), and Australian Research Council Discovery Early Career Researcher Award (DE210100908). The authors declared no conflicts of interest. The University of Adelaide provided research scholarships and laboratory access.
Publication Information
The paper, “Thi Diem Nguyen, Alexander A. T. Johnson, Enzo Lombi, Casey L. Doolette, Euan Smith, Stephanie J. Watts-Williams,” was published in Plants, People, Planet journal on July 23, 2025. It was authored by Thi Diem Nguyen, Alexander A.T. Johnson, Enzo Lombi, Casey L. Doolette, Euan Smith, and Stephanie J. Watts-Williams. The paper was received December 1, 2024, revised May 4, 2025, and accepted May 22, 2025. DOI: 10.1002/ppp3.70051.







